Abstract. Antisense oligonucleotides provide a promising approach to investigating gene function in vivo, but their ability to offer unambiguous insights into phenotypes has been debated. The recent use of morpholino antisense oligonucleotides in zebrafish embryos may prove a major advance, but rigorous controls are essential. The escalating Antisense therapy involves downregulation of gene expression by complementary oligonucleotide binding to target mRNA. Antisense oligonucleotides are short single-stranded DNA sequences engineered to be complementary to the specific ‘sense’ (5' to 3') orientation of mRNA coding for the targeted protein. After introduction into a host cell They carry genetic material, either DNA or RNA, encapsulated in a protein coat. Among RNA viruses, there are two types based on their genome type - positive and negative sense RNA viruses. Positive sense RNA viruses are also known as sense strand or plus-strand, and negative sense RNA viruses are referred to as antisense or minus strand. Phosphorodiamidate morpholino oligomers (PMO) are short single-stranded DNA analogs that are built upon a backbone of morpholine rings connected by phosphorodiamidate linkages. As uncharged nucleic acid analogs, PMO bind to complementary sequences of target mRNA by Watson–Crick base pairing to block protein translation through steric blockade. PMO interference of viral protein translation The first antisense oligonucleotides (ASOs) emerged in this context. ASOs are single-stranded synthetic RNA or DNA molecules with a mean length of 12 to 25 nucleotides. Their sequences are complementary to that of their target, to ensure specificity. The sequence of the ASO is, therefore, dictated by the sequence of its target. Antisense oligonucleotide (ASO) drugs are a type of chemically modified DNA that can be injected into cerebrospinal fluid in order to enter brain cells and reduce the amount of RNA from a specific gene. The brain is a complex mixture of hundreds of billions of cells. I am just not sure whether to use repair oligo in sense or antisense orientation with sense sgRNA. Thorben, to my knowledge it matters when you have an intact gRNA recognition sequence in your apc56F.

dna sense vs antisense